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X-gal is included in the growth medium on which cells transformed with bacterial plasmids are grown. The reason x-gal is included is to _______.

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Identify bacteria that contain a recombinant plasmid

Ampicillin is an antibiotic that stops E. coli from building cell walls, killing the bacteria in the process. The presence of the ampicillin-resistance gene causes the development of an enzyme that prevents the action of ampicillin, allowing the bacteria to survive

What is Plasmid DNA ?

Bacteria and certain other microscopic species contain plasmids, which are tiny circular DNA molecules. Physically distinct from chromosomal DNA, plasmids multiply on their own.

  • Only a small number of genes are carried by plasmids, which are separate from chromosomes, the main cell structures that house DNA. Plasmids can be taken up from the environment and passed between bacteria since they have the ability to self-replicate. The host organism makes use of plasmids to deal with situations brought on by stress.

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